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Registros recuperados: 69
Primeira ... 1234 ... Última
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A note on stability in food matrices of Salmonella enterica serovar Enteritidis-controlling bacteriophages Electron. J. Biotechnol.
Robeson,James; Turra,Gabriel; Huber,Karen; Borie,Consuelo.
Background Lytic bacteriophages are bacterial viruses that upon infection kill their host cells and therefore have re-emerged as biological control agents of bacterial pathogens, particularly in the field of food related infections. Here, we investigated the stability in different food matrices of five phage isolates capable of controlling the foodborne pathogen Salmonella enterica serovar Enteritidis (SE). Results We found that two phages, originally isolated from food sources, were up to 5 logs more stable than three phages isolated from sewage, in ten food matrices (fresh and processed) at both 4°C and 18°C. Conclusion Lytic phages isolated from contaminated food sources seem to be a better choice when structuring phage cocktails to be used in the...
Tipo: Journal article Palavras-chave: Biocontrol; Foodstuffs; Phage.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400008
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Actividad antifúngica e identificación molecular de cepas nativas de Bacillus subtilis Agrociencia
Ruiz-Sánchez,Esaú; Mejía-Bautista,Miguel Ángel.; Serrato-Díaz,Alejandra; Reyes-Ramírez,Arturo; Estrada-Girón,Yokiushirdhilgilmara; Valencia-Botín,Alberto J..
Resumen: La bioprospección de bacteria antagónicas, como agentes de control biológico para el manejo de hongos fitopatógenos, es una actividad preponderante para reducir el uso de fungicidas químicos. La bacteria Bacillus subtilis ha mostrado una excelente capacidad para inhibir el crecimiento y esporulación de una gama amplia de hongos fitopatógenos. Las características bioquímicas para diferenciar entre subespecies de B. subtilis varían a nivel regional. En esta investigación se aislaron cepas de Bacillus de la Península de Yucatán en México y se evaluaron sus actividades antagónicas por confrontación directa en un diseño completamente al azar. Para identificar las cepas más activas se realizó una caracterización molecular. Para una identificación...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Biocontrol; Secuencia gen-proteína; DNA girasa; RNA polimerasa.
Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000200133
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Antagonism of yeasts to Xanthomonas campestris pv. campestris on cabbage phylloplane in field Rev. Microbiol.
Assis,Sayonara M.P.; Mariano,Rosa L.R.; Michereff,Sami J.; Silva,Gil; Maranhão,Elizabeth A.A..
Twenty yeast isolates, obtained from cabbage phylloplane, were evaluated for antagonistic activity against Xanthomonas campestris pv. campestris, in field. Plants of cabbage cv. Midori were pulverized simultaneously with suspensions of antagonists and pathogen. After 10 days, plants were evaluated through percentage of foliar area with lesions. Percentage of disease severity reduction (DSR%) was also calculated. Yeast isolates LR32, LR42 and LR19 showed, respectively, 72, 75 and 79% of DSR. These antagonists were tested in seven different application periods in relation to pathogen inoculation (T1=4 d before; T2=simultaneously; T3=4 d after; T4=4 d before + simultaneously; T5=4 d after + simultaneously; T6=4 d before + 4 d after; T7=4 d before +...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xanthomonas campestris pv. campestris; Cabbage; Biocontrol; Yeasts.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300002
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Antagonismo in vitro de cepas bacterianas frente a Macrophomina phaseolina Tassi (Goid) Rev. Protección Veg.
Cruz-Martín,Mileidy; Sánchez-García,Cynthia; Acosta-Suárez,Mayra; Leiva-Mora,Michel; Hernández Durán,Yosbel; Roque,Berkis; Alvarado-Capó,Yelenys.
Se evaluó el control in vitro de Burkholderia cepacia (cepa CCIBP-E-556), Serratia plymuthica (cepa ATCC 1270) y Bacillus subtilis (cepa ATCC 6051), sobre Macrophomina phaseolina (Tassi) Goid, utilizando el método de cultivo dual.Se midió el radio de la colonia del hongo y se determinó el porcentaje de inhibición del crecimiento radial. B. cepacia produjo el mayor efecto sobre el aislado de M. phaseolina, provocando un porcentaje de inhibición del 43,5%, y ocasionando cambios en la apariencia del micelio, que se observaron en la zona de inhibición con el uso del microscópico óptico. Por su parte, S. plymuthica y B. subtilis provocaron valores de inhibición del crecimiento micelial en M. phaseolina por debajo del 10%, lo que indica que dichas cepas no...
Tipo: Journal article Palavras-chave: Actividad antifúngica; Biocontrol; Burkholderia cepacia; Serratia plymuthica; Bacillus subtilis; Frijol; Phaseolus vulgaris; Fitopatógeno.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100009
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Antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives BJM
Garcia,Roxana; Alves,Eliomara S.S.; Santos,Mirella P.; Aquije,Glória M.F. Viégas; Fernandes,A. Alberto R.; Santos,Reginaldo B. dos; Ventura,Jose A.; Fernandes,Patricia M.B..
Banana, papaya and pineapple are the most consumed tropical fruits in the world, being Brazil one of the main producers. Fungi Colletotrichum musae, Colletotrichum gloeosporioides and Fusarium subglutinans f.sp. ananas cause severe post harvest diseases and losses in fruits quality. The aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial growth and conidia germination of these three phytopathogens. The monoterpenes citral, citronellal, L-carvone, isopullegol and a-pinene were diluted in ethanol to final concentrations from 0.2 to 1%. All monoterpenes were found to inhibit the growth of the three studies fungi in a dose-dependent manner. Citral was the most effective of the oils tested and showed potent fungicidal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Essential oils; Banana; Papaya; Pineapple; Plant diseases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100032
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Biocontrol potential of saline- or alkaline-tolerant Trichoderma asperellum mutants against three pathogenic fungi under saline or alkaline stress conditions BJM
Guo,Ruiting; Wang,Zhiying; Huang,Ying; Fan,Haijuan; Liu,Zhihua.
ABSTRACT Salinity and alkalinity are major abiotic stresses that limit growth and development of poplar. We investigated biocontrol potential of saline- and alkaline-tolerant mutants of Trichoderma asperellum to mediate the effects of salinity or alkalinity stresses on Populus davidiana × P. alba var. pyramidalis (PdPap poplar) seedlings. A T-DNA insertion mutant library of T. asperellum was constructed using an Agrobacterium tumefaciens mediated transformation system; this process yielded sixty five positive transformants (T1–T65). The salinity tolerant mutant, T59, grew in Potato Dextrose Agar (PDA) containing up to 10% (1709.40 mM) NaCl. Under NaCl-rich conditions, T59 was most effective in inhibiting Alternaria alternata (52.00%). The alkalinity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Trichoderma asperellum; Mutant library; Salinity and alkalinity; Fungal disease.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500236
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Bioeficacia de productos orgánicos, biológicos y químicos contra Alternaria dauci Kühn y su efecto en el cultivo de zanahoria Phyton
Hernández-Castillo,FD; Aguirre-Aguirre,Adalberto; Lira-Saldivar,RH; Guerrero-Rodríguez,E; Gallegos-Morales,G.
Mediante experimentos de laboratorio e invernadero se analizó la bioeficacia de cinco cepas de Bacillus spp.; de la mezcla de los bioproductos quitosan y extracto de Larrea tridentata, así como de cinco funguicidas sintéticos contra el hongo Alternaria dauci Kühn. Además, se determinó el efecto de estos compuestos en el crecimiento del cultivo de zanahoria. Los resultados obtenidos mostraron que las cepas de la bacteria Bacillus (B3, B9, B15 y la combinación de éstas), así como la mezcla quitosan- Larrea estimularon el desarrollo de la zanahoria, ya que ésta alcanzó mayor grosor, longitud y peso, con respecto al testigo y a los demás tratamientos. La cepa B3 resultó ser la que más promovió el crecimiento de zanahoria, ya que reportó valores superiores en...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Biocontrol; Quitosan; Daucus carota; Larrea tridentata.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572006000100009
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Biological control of mycotoxin-producing molds Ciência e Agrotecnologia
Medeiros,Flávio Henrique Vasconcelos de; Martins,Samuel Julio; Zucchi,Tiago Domingues; Melo,Itamar Soares de; Batista,Luis Roberto; Machado,José da Cruz.
Mycotoxins are produced by the secondary metabolism of many fungi and can be found in almost 25% of the world's agricultural commodities. These compounds are toxic to humans, animals, and plants and therefore, efforts should be made to avoid mycotoxin contamination in food and feed. Besides, up to 25% of all harvested fruits and vegetables are lost due to storage molds and/or mycotoxin contamination and many methods have been applied to mitigate these issues, but most of them rely on the use of fungicides. Although chemicals are often the first defensive line against mycotoxigenic fungi, the indiscriminate use of fungicides are awakening the public perception due to their noxious effects on the environment and human/animal health. Thus, there is an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Microbial antagonists; Postharvest decay; Food safety.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000500001
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Biological control of rice brown spot with native isolates of three Trichoderma species BJM
Khalili,Elham; Sadravi,Mehdi; Naeimi,Shahram; Khosravi,Vahid.
Brown spot caused by Bipolaris oryzae is an important rice disease in Southern coast of Caspian Sea, the major rice growing region in Iran. A total of 45 Trichoderma isolates were obtained from rice paddy fields in Golestan and Mazandaran provinces which belonged to Trichoderma harzianum, T. virens and T. atroviride species. Initially, they were screened against B. oryzae by antagonism tests including dual culture, volatile and nonvolatile metabolites and hyperparasitism. Results showed that Trichoderma isolates can significantly inhibit mycelium growth of pathogen in vitro by producing volatile and nonvolatile metabolites Light microscopic observations showed no evidence of mycoparasitic behaviour of the tested isolates of Trichoderma spp. such as coiling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rice brown spot; Biocontrol; Trichoderma harzianum; T. virens; T. atroviride.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100035
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Bioprospecting endophytic bacteria for biological control of coffee leaf rust Scientia Agricola
Shiomi,Humberto Franco; Silva,Harllen Sandro Alves; Melo,Itamar Soares de; Nunes,Flávia Vieira; Bettiol,Wagner.
Suppression of plant diseases due to the action of endophytic microorganisms has been demonstrated in several pathosystems. Experiments under controlled conditions involving endophytic bacteria isolated from leaves and branches of Coffea arabica L and Coffea robusta L were conducted with the objective of evaluating the inhibition of germination of Hemileia vastatrix Berk. & Br., race II, urediniospores and the control of coffee leaf rust development in tests with leaf discs, detached leaves, and on potted seedling of cv. Mundo Novo. The endophytic bacterial isolates tested proved to be effective in inhibiting urediniospore germination and/or rust development, with values above 50%, although the results obtained in urediniospore germination tests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemileia vastatrix; Coffea spp.; Bacillus; Biocontrol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000100006
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Biosurfactants synthesized by endophytic Bacillus strains as control of Moniliophthora perniciosa and Moniliophthora roreri Scientia Agricola
Serrano,Lizette; Moreno,Andrea Sosa; Castillo,Daynet Sosa Del; Bonilla,Julio; Romero,Christian A; Galarza,Luis Lenin; Coronel–León,Jonathan Ricardo.
ABSTRACT: Ecuador is responsible for up to 70 % of the world’s production of fine flavor cocoa. Although fungal diseases have reduced cocoa production, eco–friendly alternatives have been implemented to control the spread of phytopathogens. This study aimed to explore the potential of endophytic strains isolated from fine flavor Nacional cocoa trees to synthetize compounds with antimicrobial and surface active properties. It was determined that under in vitro conditions, seven strains (DS03, DS07, DS18, DS23, DS31, DS34 and DS50) exhibited antifungal activity that inhibited the mycelial growth of Moniliophthora roreri and Moniliophthora perniciosa. The strains were identified based on their morphological (pattern forming Bacilli) and genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial biosurfactant; Biocontrol; Cocoa; Endophytes microorganisms.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000700201
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Braconidae (Hymenoptera: Ichneumonoidea) Collected in a Native Forest Area in Itumbiara, Goiás, Brazil Neotropical Entomology
MARCHIORI,CARLOS H.; PENTEADO-DIAS,ANGÉLICA M..
This research work was carried out in order to identify the genera of Braconidae (Hymenoptera) collected in a remnant area of native forest in Itumbiara County, State of Minas Gerais, Brazil, using Malaise traps. The Malaise trap captures the insects by interception. After 24 sample collections, a total of 49 specimens from 19 different genera and 10 subfamilies were obtained. The most frequent genus was Chelomus with 34.7% frequency. March and June were the months of highest occurrence of Braconidae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Parasitoid; Natural enemy; Malaise trap.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000400020
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Caenorhabditis elegans as an indicator of toxicity of Bacillus thuringiensis strains to Meloidogyne incognita race 3 Ciência Rural
Montalvão,Sandro Coelho Linhares; Castro,Marcelo Tavares de; Soares,Carlos Marcelo Silveira; Blum,Luiz Eduardo Bassay; Monnerat,Rose Gomes.
ABSTRACT: The cotton plant (Gossypium hirsutum) is affected by several diseases of economic importance, among them root-knot nematode (Meloidogyne incognita races 3 and 4). Methods to control this disease include the application of nematicides, solarization, deep plowing, crop rotation and use of antagonistic microorganisms. Among species of Bacillus, there are strains that act as bioregulators and antagonists of several pathogens. Tests to identify these strains are hampered by the difficulty of obtaining large populations of the pathogen and by the time of execution of the in vivo tests that should be conducted for about 90 days. The objective of this research was to compare the toxicity of B. thuringiensis strains to two nematodes, M. incognita and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Phytonematode; Root-knot nematode; Rhizobacteria.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700151
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Control of Listeria monocytogenes growth in soft cheeses by bacteriophage P100 BJM
Silva,Elaine Nóbrega Gibson; Figueiredo,Ana Cláudia Leite; Miranda,Fernanda Araújo; Almeida,Rogeria Comastri de Castro.
The purpose of this study was to determine the effect of bacteriophage P100 on strains of Listeria monocytogenes in artificially inoculated soft cheeses. A mix of L. monocytogenes 1/2a and Scott A was inoculated in Minas Frescal and Coalho cheeses (approximately 10(5) cfu/g) with the bacteriophage added thereafter (8.3 x 10(7) PFU/g). Samples were analyzed immediately, and then stored at 10 ºC for seven days. At time zero, 30 min post-infection, the bacteriophage P100 reduced L. monocytogenes counts by 2.3 log units in Minas Frescal cheese and by 2.1 log units in Coalho cheese, compared to controls without bacteriophage. However, in samples stored under refrigeration for seven days, the bacteriophage P100 was only weakly antilisterial, with the lowest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food safety; Listeria; Biocontrol; Bacteriophage; Cheese.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100003
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Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. BJM
Turatto,Marcielly F.; Dourado,Fernanda dos S.; Zilli,Jerri E.; Botelho,Glória R..
ABSTRACT Plant Growth Promoting Rhizobacteria (PGPR) have different mechanisms of action in the development of plants, such as growth promotion, production of phytohormones and antibiotic substances and changes in root exudates. These help to control plant diseases. In order to evaluate the potential of microorganisms in the control of Meloidogyne javanica and Ditylenchus spp., five rhizobacteria isolated from rhizosphere of garlic cultivated in the Curitibanos (SC) region were tested. Hatching chambers were set on Petri dishes, in which were added 10 mL of bacterial suspension and 1 mL of M. javanica eggs suspension, at the rate of 4500, on the filter paper of each chamber. The same procedure was performed with 300 juvenile Ditylenchus spp. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium sativum L.; Glycine max (L.) Merril; Biocontrol; Plant parasitic nematodes; Rhizobacteria.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100054
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Distribución geográfica, prevalencia e intensidad de las infecciones de Gregarina ronderosi (Eugregarinorida: Gregarinidae) en Dichroplus elongatus (Orthoptera: Acrididae) Rev. Soc. Entomol. Argent.
Bardi,Christian; Lange,Carlos E..
Gregarina ronderosi es un parásito obligado del tracto digestivo del acrídido plaga Dichroplus elongatus y una de las únicas dos eugregarinas de acrídidos argentinos descriptas con su ciclo de vida completo. Dada la falta de conocimiento acerca de aspectos epizootiológicos fundamentales de las infecciones causadas por eugregarinas en acrídidos de Argentina, el objetivo de esta contribución es iniciar el registro de la distribución geográfica de G. ronderosi en la región Pampeana, su prevalencia natural y la intensidad de las infecciones en condiciones naturales. Para ello, se colectaron ejemplares de D. elongatus (2008 - 2012) en distintos puntos de la región Pampeana. Se obtuvieron y analizaron un total de 4084 ejemplares provenientes de cuarenta y dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melanoplinae; Biocontrol; Apicomplexa; Tucura; Plaga.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802016000100001
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Diversidad y caracterización de poblaciones bacterianas asociadas a esclerocios de Claviceps gigantea Fuentes et al. en maíz (Zea mays L.) con potencial antagonista, en el Estado de México. Colegio de Postgraduados
Ayala Torres, Ana María.
Un total de 129 aislamientos bacterianos obtenidos de la micósfera de esclerocios de Claviceps gigantea se evaluaron in vitro para determinar su capacidad de inhibir el crecimiento de Fusarium graminearum, F. subglutinans y F. verticillioides patógenos a maíz. Ensayos cualitativos en confrontación dual, resultaron en la selección de 22 aislamientos bacterianos con el mayor potencial antagónico hacia una, dos o las tres especies de Fusarium. Los aislamientos se identificaron molecularmente mediante secuenciación parcial del gen 16s ADNr. Las cepas con mayor grado de antagonismo pertenecen a los géneros Bacillus y Pseudomonas. La caracterización en la producción de metabolitos potencialmente involucrados en la actividad antagónica, mostró que el 100% de las...
Palavras-chave: Bacillus; Pseudomonas; Biocontrol; Metabolitos secundarios; Caracterización; Secondary metabolites; Characterization; Fitopatología; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2299
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DNA barcodes, expanded distribution, and redescription of Apanteles hemara Nixon, 1965 (Hymenoptera, Braconidae, Microgastrinae), a potential biocontrol species against amaranth leaf-webbers in Africa Naturalis
Fernandez-Triana, J.; Beaudin, M.; Achterberg, K. van; Agbodzavu, M.K.; Othim, S.T.O.; Nyamu, F.W.; Fiaboe, K.K.M..
The microgastrine parasitoid wasp Apanteles hemara Nixon, 1965, is currently being considered as a potential biocontrol agent of amaranth leaf-webber pests in Africa. To facilitate future research and identification of the species, we characterize it from an integrative taxonomy perspective by providing a comprehensive morphological redescription, extensive illustrations (including the first images of the holotype), DNA barcodes, wasp biology, host data (Choreutidae and Crambidae caterpillars), and updated geographical distribution of the species (including eight new country records). Despite a wide distribution across four major biogeographical regions (mostly within the Old World tropics), the species seems to be relatively uniform from a molecular and...
Tipo: Article / Letter to the editor Palavras-chave: Microgastrinae; Amaranthus; Biocontrol; DNA barcode; Geographical distribution; Africa.
Ano: 2017 URL: http://www.repository.naturalis.nl/record/643636
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Ecological, phytosanitary, and agronomic aspects of target weeds for biological control studies in Antioquia, Colombia Acta Agron. (Palmira)
Mira Taborda,Yerly Dayana; Castañeda Sánchez,Darío Antonio; Morales Osorio,Juan Gonzalo; Patiño Hoyos,Luis Fernando.
ABSTRACT Biological control as part of integrated weed management represents an emerging and promising method to minimize the use and impact of herbicides on the environment. The objective of this research was to determine the ecological, phytosanitary, and agronomic status of weeds associated with crops in Antioquia, Colombia, and their potential to be chosen as candidates for biological control studies using pathogenic fungi. The research was carried out through a sampling of weeds in 35 agricultural farms, distributed in 11 municipalities and 10 different crops, on which population ecological variables such as frequency and dominance were evaluated; phytosanitary variables such as incidence and severity of foliar fungal symptoms; as well as some...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Phytosociology; Weed ecology; Weed biology; Weed management.
Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122022000200195
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Effect of Bacillus thuringiensis on microbial functional groups in sorghum rhizosphere PAB
Brasil,Carlos; Matsumoto,Leopoldo Sussumu; Nogueira,Marco Antonio; Spago,Flavia Regina; Rampazo,Luís Gustavo; Cruz,Marcio Ferreira; Andrade,Galdino.
The objective of this work was to assess the effect of two strains of Bacillus thuringiensis var. kurstaki on sorghum rhizosphere microorganisms. The strains were HD1, that produces the bioinsecticidal protein, and 407, that is a mutant non-producer. The strains do not influence microbial population, but reduce plant growth and improve mycorrhizal colonization and free living fixing N2 community.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bioinsecticide; Sorgum; Rhizosphere; Biocontrol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000500022
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